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Differentiation of 3T3-L1 pre-adipocytes 3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work.[18] Briefly, standard culture media was supplemented with scheduled addition of adipogenic agents [3-isobutyl-1methyl xanthine (IBMX), Sigma Aldrich http://cosmocosmetics.com/cdn/shop/files/gluta-papaya-BODY-SCRUB-1.jpg?v=1740464631 Essential Fx Acyl-Glutathione Intensive Overnight Moisturizer free from:Gluten Style:Soap
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