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Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP https://www.journalofdairyscience.org/cms/10.3168/jds.2024-25167/asset/5cfd30d3-a23e-48aa-b55d-d169834f44c1/main.assets/fx1_lrg.jpg Evexias BPC-157 type1:Cell Viability Assays > Oxidative Stress Treatment:3 Months

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